Re: '05 5.3l mysterious misfire
HPTuners scanner will allow you to log each cylinder for a misfire so you can isolate the cylinder. Why did your tuner turn off the misfire detection? Swap your injectors side to side and see if you see any difference. Autozone and Advance auto sell the infared thermometers at a decent price. I would also recheck all of your grounds and coil pack wiring for connection and wire breaks.
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